Ordering Recommendation

Use to determine percentage of hemoglobin F. For complete hemoglobin evaluation, order Hemoglobin Evaluation With Reflex to Electrophoresis and/or RBC Solubility (3017101).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Lavender (EDTA) or pink (K2EDTA).

Specimen Preparation

Transport 3 mL whole blood. (Min: 0.2 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Frozen or room temperature specimens.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable

Methodology

High Performance Liquid Chromatography (HPLC)/Electrophoresis

Performed

Sun-Sat

Reported

1-3 days

Reference Interval

Age-Defined Normal Hemoglobin Reference Intervals

Age Hgb F Percent
0-1 month 45.8-91.7
2 months 32-7-85.2
3 months 14.5-73.7
4 months 4.2-56.9
5 months 1.0-38.1
6-8 months 0.9-19.4
9-12 months 0.6-11.6
13-23 months 0.0-8.5
2 years and older 0.0-2.1

Interpretive Data

Refer to report.

Compliance Category

FDA

Note

This assay measures percentage of hemoglobin F only. If abnormal peaks suggestive of a hemoglobin variant are detected, then Capillary Electrophoresis will be added to aid in confirmation and identification of the variant. Additional charges apply. For complete hemoglobin evaluation, order Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility (0050610).

Hotline History

N/A

CPT Codes

83021; if reflexed, add 83020

Components

Component Test Code* Component Chart Name LOINC
2008793 Hemoglobin, Capillary Electrophoresis 13514-5
3002710 Hemoglobin F 4576-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

Hemoglobin F with Reflex to Electrophoresis